Microbiology and unknown bacteria lab

Neurophysiology Virtual Lab pilot Neurophysiology is the study of nervous system function. Primarily, it is connected with neurobiology, psychology, neurology, clinical neurophysiology, electrophysiology, biophysical neurophysiology, ethology, neuroanatomy, cognitive science and other brain sciences.

Microbiology and unknown bacteria lab

Printer friendly version [26 pages] Microbiology laboratories commonly receive cerebrospinal fluid CSF or blood specimens from patients with meningitis, pneumonia, or unexplained febrile illness. Laboratories may also receive joint fluid, pleural fluid, or other sterile site specimens from these patients.

Presumptive identification of N. Methods for confirmatory identification of N.

CAR bacillus

Identification and Characterization of N. Identification and Characterization of S. Identification and Characterization of H. Personnel who are at risk of routine exposure to aerosolized N.

Additional health and safety information can be found in Chapter 4: While laboratory-acquired infections with S. Because the primary purpose of this manual is to aid in the identification of N. Processing CSF specimens A note about centrifugation: RCF is dependent on the length of the radius of the rotor, thus the same RPM may not generate the same g force in another centrifuge.

Therefore, RCF should be used to describe the centrifuge speed. Typically, centrifugation at x g for minutes is sufficient to sediment bacteria. After the specimen has been centrifuged, the supernatant should be drawn off with a Pasteur pipette and reserved if antigen detection by latex agglutination is planned.

The sediment should be vigorously mixed e. Once it has been well-mixed, one or two drops of sediment should be used to prepare the Gram stain and one drop should be used to streak the primary culture media.

Microbiology and unknown bacteria lab

Cytological examination of the CSF Laboratory examination of the CSF is usually the first step to confirm the presence of bacterial meningitis. Note that cytological examination should precede centrifugation and heating of the CSF.

Typical CSF abnormalities associated with bacterial meningitis include the following: Presumptive identification by Gram stain, latex agglutination, or rapid diagnostic test RDT In combination with a clinical picture and CSF examination consistent with bacterial meningitis, a presumptive diagnosis of bacterial meningitis caused by N.

Positive results for any of these tests can rapidly provide evidence of infection, even if cultures fail to grow.Learn microbiology lab identification unknowns with free interactive flashcards.

Choose from different sets of microbiology lab identification unknowns flashcards on Quizlet. In this lab, students identify an unknown bacteria using a biochemical method and a molecular method. For the biochemical method, students use a combination of differential growth tests and enzyme tests developed for clinical use.

For the molecular method, students PCR amplify and sequence the 16S. Free microbiology lab teaching materials on identification of bacteria: differential staining (Gram, Acid-fast, Endospore) & specialized media. The identification of bacteria is a careful and systematic process that uses many different techniques to narrow down the types of bacteria that are present in an unknown bacterial culture.

Special offers

It produces benefits for many aspects of the research of microorganisms and helps physicians correctly treat patients. UNKNOWN LAB REPORT. Unknown Number (Staphylococcus aureus and Klebsiella pneumoniae) Michelle Gudorp General Microbiology.

Spring INTRODUCTION. It is important to understand why a person would want to identify between different bacteria. Learn the basic differences. There are key differences between bacteria and viruses in size, origins, and effects on the body.

Viruses are the smallest and simplest life form; they are 10 to times smaller than bacteria.

Microbiology and unknown bacteria lab
Biol. Lab Manual - Table of Contents